How you process your samples directly impacts which drying accessories are needed for your freeze dryer. The samples would be unable to maintain their frozen state and the preservation properties of sublimation would be lost because the samples would begin to melt and evaporate.ĭepicted: Slant freezing (left), shell freezing (center) and stub freezing (right)ģ. This would result in poor vacuum, an increase in collector temperature and a low recovery of solvent on the coils. If you were to put 6 liters of volume on the freeze dryer you would overload the system. For example, a 6L freeze dryer will be able to process about 3 liters of sample volume per run. ![]() Generally, Labconco FreeZone Freeze Dryers handle a sample volume approximately half of the total ice holding capacity of the freeze dryer. What is your total sample volume per run? In these cases, PTFE coating of the collector and coils is recommended to prevent rust and other irreparable damage.Ģ. Even in small concentrations, acids or other harsh solvents can damage stainless steel over prolonged use. It is also important to consider whether any of your samples are corrosive to stainless steel. If the collector is not cold enough the vapor will bypass the coil and enter the vacuum pump, potentially causing damage. In order to effectively trap the vapor coming off your samples, you need a temperature differential of 15-20⁰ between the sample’s freezing point and the collector coil of your freeze dryer. Knowing what components make up your samples is important. In order to choose the correct lyophilizer for your sample throughput, consider the following questions:ġ. This vapor is then collected on a cold coil in the form of ice. Using the process of sublimation, wherein a sample changes from a solid to a gas, moisture is removed from the sample using heat and a deep vacuum. Lyophilization, commonly referred to as freeze drying, is used to process samples for long term storage or as a sample prep step.
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